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XIAP ELISA Kit
XIAP
Reactivity: Human, Mouse
Colorimetric
Sandwich ELISA
0.25-600 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details XIAP ELISA Kit
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Target
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XIAP
(X-Linked Inhibitor of Apoptosis (XIAP))
Reactivity
All reactivities for XIAP elisa kits
Human, Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.25-600 ng/mL
Minimum Detection Limit
0.25 ng/mL
Application
ELISA
Purpose
Human XIAP ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair can recognize human and mouse XIAP.
Sensitivity
250 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for XIAP
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Target
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XIAP
(X-Linked Inhibitor of Apoptosis (XIAP))
Alternative Name
XIAP (XIAP Products )
Synonyms
API3 ELISA Kit, BIRC4 ELISA Kit, IAP-3 ELISA Kit, ILP1 ELISA Kit, MIHA ELISA Kit, XLP2 ELISA Kit, hIAP-3 ELISA Kit, hIAP3 ELISA Kit, api3 ELISA Kit, birc4 ELISA Kit, ilp1 ELISA Kit, miha ELISA Kit, xlp2 ELISA Kit, xxiap ELISA Kit, 1110015C02Rik ELISA Kit, Aipa ELISA Kit, Api3 ELISA Kit, Birc4 ELISA Kit, IAP3 ELISA Kit, ILP-1 ELISA Kit, mXIAP ELISA Kit, riap3 ELISA Kit, fb73d01 ELISA Kit, wu:fb73d01 ELISA Kit, zgc:91873 ELISA Kit, BIRC8 ELISA Kit, X-linked inhibitor of apoptosis ELISA Kit, X-linked inhibitor of apoptosis L homeolog ELISA Kit, XIAP ELISA Kit, xiap ELISA Kit, xiap.L ELISA Kit, Xiap ELISA Kit
Background
Gene Names: XIAP API3 BIRC4 IAP3 Protein names: E3 ubiquitin-protein ligase XIAP (EC 6.3.2.-) (Baculoviral IAP repeat-containing protein 4) (IAP-like protein) (ILP) (hILP) (Inhibitor of apoptosis protein 3) (IAP-3) (hIAP-3) (hIAP3) (X-linked inhibitor of apoptosis protein) (X-linked IAP)
Gene ID
331
UniProt
P98170
Pathways
Apoptosis , Caspase Cascade in Apoptosis , Transition Metal Ion Homeostasis
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