HTRA2 ELISA Kit

Catalog No. ABIN625463

Product Details HTRA2 ELISA Kit

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.2-50 ng/mL

Minimum Detection Limit: 0.2 ng/mL

Application: ELISA

Purpose: Human HTRA2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detect human HtrA2. Other species not determined yet.

Sensitivity: 200 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for HTRA2

Target: HTRA2 (HtrA Serine Peptidase 2 (HTRA2))

Alternative Name: HTRA2 (HTRA2 Products)

Synonyms: CG8464 ELISA Kit, DmHtrA2 ELISA Kit, Dmel\\CG8464 ELISA Kit, HtrA2/Omi ELISA Kit, Omi ELISA Kit, dOmi ELISA Kit, domi ELISA Kit, htrA2 ELISA Kit, omi ELISA Kit, HTRA2 ELISA Kit, PRSS25 ELISA Kit, OMI ELISA Kit, PARK13 ELISA Kit, AI481710 ELISA Kit, Prss25 ELISA Kit, mnd2 ELISA Kit, HTRA2-related serine protease ELISA Kit, HtrA serine peptidase 2 ELISA Kit, HtrA2 ELISA Kit, HTRA2 ELISA Kit, htra2 ELISA Kit, Htra2 ELISA Kit

Background: Gene Names: HTRA2 OMI PRSS25
Protein names: Serine protease HTRA2, mitochondrial (EC 3.4.21.108) (High temperature requirement protein A2) (HtrA2) (Omi stress-regulated endoprotease) (Serine protease 25) (Serine proteinase OMI)

Gene ID: 27429

UniProt: O43464

Pathways: Positive Regulation of Endopeptidase Activity