AGRN ELISA Kit

Catalog No. ABIN625443

Product Details AGRN ELISA Kit

Target: AGRN (Agrin (AGRN))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.06-50 ng/mL

Minimum Detection Limit: 0.06 ng/mL

Application: ELISA

Purpose: Rat Agrin ELISA Kit for Cell Culture Supernatants, Heparin and/or EDTA treated plasma, and Serum samples. Citrate is not recommended.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes rat Agrin.

Sensitivity: 0.06 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Sample Preparation:

Collect plasma using EDTA and/or Heparin as the anticoagulant. Citrate is not recommended.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for AGRN

Target: AGRN (Agrin (AGRN))

Alternative Name: Agrin (AGRN Products)

Synonyms: AGRIN ELISA Kit, agrn ELISA Kit, wu:fb57e07 ELISA Kit, Agrin ELISA Kit, nmf380 ELISA Kit, AGR ELISA Kit, HSPG ELISA Kit, agrin ELISA Kit, LOC465392 ELISA Kit, agrn ELISA Kit, AGRN ELISA Kit, Agrn ELISA Kit

Background: Gene Names: Agrn Agrin
Protein names: Agrin [Cleaved into: Agrin N-terminal 110 kDa subunit, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 90 kDa fragment (C90), Agrin C-terminal 22 kDa fragment (C22)]

Gene ID: 25592

UniProt: P25304

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development