CCL9 ELISA Kit

Catalog No. ABIN625420

Product Details CCL9 ELISA Kit

Target: CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1-250 pg/mL

Minimum Detection Limit: 1 pg/mL

Application: ELISA

Purpose: Mouse MIP-1 gamma ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 delta, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

Sensitivity: 1 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples40 - 400 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for CCL9 ELISA Kit (ABIN625420)

Li, Zhou, Zhang, Zheng, He, Ye, Zhang, Qi, He, Yu, Shao, Qiao, Wang: "Inflammatory Molecule,PSGL-1, Deficiency Activates Macrophages to Promote Colorectal Cancer Growth through NFκB Signaling." in: Molecular cancer research : MCR, Vol. 15, Issue 4, pp. 467-477, (2017) (PubMed).

Gong, Liu, Wu, Qi, Wu, Shu, Li, Chen, Wang, Li, Tang, Ji, Yuan, Yao, Shang: "MARESIN 1 PREVENTS LIPOPOLYSACCHARIDE-INDUCED NEUTROPHIL SURVIVAL AND ACCELERATES RESOLUTION OF ACUTE LUNG INJURY." in: Shock (Augusta, Ga.), Vol. 44, Issue 4, pp. 371-80, (2015) (PubMed).

Huggins, Pearce, Peri, Neumann, Cockerill, Pirianov: "A novel small molecule TLR4 antagonist (IAXO-102) negatively regulates non-hematopoietic toll like receptor 4 signalling and inhibits aortic aneurysms development." in: Atherosclerosis, Vol. 242, Issue 2, pp. 563-70, (2015) (PubMed).

Shanmugam, Wang, Markand, Perry, Tawfik, Zorrilla, Ganapathy, Smith: "Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells." in: Journal of neurochemistry, (2015) (PubMed).

Target Details for CCL9

Target: CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))

Alternative Name: MIP-1 gamma (Ccl9 Products)

Synonyms: CCF18 ELISA Kit, MRP-2 ELISA Kit, Scya10 ELISA Kit, Scya9 ELISA Kit, chemokine (C-C motif) ligand 9 ELISA Kit, Ccl9 ELISA Kit

Background: Gene Names: Ccl9 Mrp2 Scya10 Scya9
Protein names: C-C motif chemokine 9 (CCF18) (Macrophage inflammatory protein 1-gamma) (MIP-1-gamma) (Macrophage inflammatory protein-related protein 2) (MRP-2) (Small-inducible cytokine A9) [Cleaved into: CCL9(29-101), CCL9(30-101), CCL9(31-101)]

Gene ID: 20308

UniProt: P51670