×
For best experience we recommend to activate Javascript in your browser.
TNFRSF10A ELISA Kit
TNFRSF10A
Reactivity: Human
Colorimetric
Sandwich ELISA
40-10.000 pg/mL
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details TNFRSF10A ELISA Kit
(hide)
Target
See all TNFRSF10A ELISA Kits
TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
Reactivity
All reactivities for TNFRSF10A elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
40-10.000 pg/mL
Minimum Detection Limit
40 pg/mL
Application
ELISA
Purpose
Custom Human TRAIL Receptor 1 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes human TRAIL R1.
Sensitivity
40 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Featured
Discover our best selling TNFRSF10A ELISA Kit
Top Product
Discover our top product TNFRSF10A ELISA Kit
Alternatives
(show)
Application Details
(hide)
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
(hide)
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for TNFRSF10A
(hide)
Target
See all TNFRSF10A ELISA Kits
TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
Alternative Name
TRAIL R1 (TNFRSF10A Products )
Synonyms
APO2 ELISA Kit, CD261 ELISA Kit, DR4 ELISA Kit, TRAILR-1 ELISA Kit, TRAILR1 ELISA Kit, TNFRSF10A ELISA Kit, TNF receptor superfamily member 10a ELISA Kit, tumor necrosis factor receptor superfamily member 26 ELISA Kit, tumor necrosis factor receptor superfamily, member 10a ELISA Kit, TNFRSF10A ELISA Kit, LOC510798 ELISA Kit
Background
Gene Names: TNFSF10 APO2L TRAIL Protein names: Tumor necrosis factor ligand superfamily member 10 (Apo-2 ligand) (Apo-2L) (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)
Gene ID
8797
UniProt
O00220
Pathways
Apoptosis , Positive Regulation of Endopeptidase Activity
Recently viewed
(hide)