Recommended Dilution for serum and plasma samples2 - 20 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 μL of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Restrictions
For Research Use only
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Nirala, Perumal, Gohil: "Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction." in: Diabetes & vascular disease research, Vol. 12, Issue 4, pp. 290-7, (2015) (PubMed).
Nirala, Gohil: "Effect of garlic component s-allyl cysteine sulfoxide on glycated human serum albumin induced activation of endothelial cells: an in vitro study." in: European review for medical and pharmacological sciences, Vol. 19, Issue 11, pp. 2125-31, (2015) (PubMed).
Bala, Gohil: "Interaction of glycated protein and DFO mimicked hypoxia in cellular responses of HUVECs." in: Molecular bioSystems, Vol. 8, Issue 10, pp. 2657-63, (2012) (PubMed).
Bala, Gomes, Gohil: "Interaction of glycated human serum albumin with endothelial cells in a hemodynamic environment: structural and functional correlates." in: Molecular bioSystems, Vol. 7, Issue 11, pp. 3036-41, (2012) (PubMed).
Chima, LaMontagne, Piraino, Hake, Denenberg, Zingarelli: "C-peptide, a novel inhibitor of lung inflammation following hemorrhagic shock." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 300, Issue 5, pp. L730-9, (2011) (PubMed).