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PRSS27 ELISA Kit
PRSS27
Reactivity: Human
Colorimetric
Sandwich ELISA
35-10000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details PRSS27 ELISA Kit
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Target
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PRSS27
(Protease, Serine 27 (PRSS27))
Reactivity
All reactivities for PRSS27 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
35-10000 pg/mL
Minimum Detection Limit
35 pg/mL
Application
ELISA
Purpose
Human Marapsin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity
35 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples3 - 30 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PRSS27
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Target
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PRSS27
(Protease, Serine 27 (PRSS27))
Alternative Name
Marapsin (PRSS27 Products )
Synonyms
PRSS27 ELISA Kit, mpn ELISA Kit, caph2 ELISA Kit, tmprss9 ELISA Kit, marapsin ELISA Kit, pancreasin ELISA Kit, MGC97879 ELISA Kit, CAPH2 ELISA Kit, MPN ELISA Kit, Mpn ELISA Kit, protease, serine 27 ELISA Kit, epidermis specific serine protease S homeolog ELISA Kit, PRSS27 ELISA Kit, prss27 ELISA Kit, xepsin.S ELISA Kit, Prss27 ELISA Kit
Background
Gene Names: PRSS27 MPN UNQ1884/PRO4327 Protein names: Serine protease 27 (EC 3.4.21.-) (Marapsin) (Pancreasin)
Gene ID
83886
UniProt
Q9BQR3
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