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PRSS27 ELISA Kit

PRSS27 Reactivity: Human Colorimetric Sandwich ELISA 35-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN625335
  • Target See all PRSS27 ELISA Kits
    PRSS27 (Protease, Serine 27 (PRSS27))
    Reactivity
    • 3
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    35-10000 pg/mL
    Minimum Detection Limit
    35 pg/mL
    Application
    ELISA
    Purpose
    Human Marapsin ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    Sensitivity
    35 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product PRSS27 ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples3 - 30 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all PRSS27 ELISA Kits
    PRSS27 (Protease, Serine 27 (PRSS27))
    Alternative Name
    Marapsin (PRSS27 Products)
    Synonyms
    PRSS27 ELISA Kit, mpn ELISA Kit, caph2 ELISA Kit, tmprss9 ELISA Kit, marapsin ELISA Kit, pancreasin ELISA Kit, MGC97879 ELISA Kit, CAPH2 ELISA Kit, MPN ELISA Kit, Mpn ELISA Kit, protease, serine 27 ELISA Kit, epidermis specific serine protease S homeolog ELISA Kit, PRSS27 ELISA Kit, prss27 ELISA Kit, xepsin.S ELISA Kit, Prss27 ELISA Kit
    Background
    Gene Names: PRSS27 MPN UNQ1884/PRO4327
    Protein names: Serine protease 27 (EC 3.4.21.-) (Marapsin) (Pancreasin)
    Gene ID
    83886
    UniProt
    Q9BQR3
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