TYRO3 ELISA Kit

Catalog No. ABIN625289

Product Details TYRO3 ELISA Kit

Target: TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 40-10,000 pg/mL

Minimum Detection Limit: 40 pg/mL

Application: ELISA

Purpose: Human Dtk ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Characteristics: Custom Human Dtk ELISA Kit available on demand

Components: Pre-Coated 96-Well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Material not included: Distilled or deionized water
100 mL and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for TYRO3

Target: TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

Alternative Name: Dtk (TYRO3 Products)

Pathways: RTK Signaling