TGFA ELISA Kit

Catalog No. ABIN625093

Product Details TGFA ELISA Kit

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3-1000 pg/mL

Minimum Detection Limit: 3 pg/mL

Application: ELISA

Purpose: Human TGF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ALCAM, Angiopoietin-1, Angiostatin, B7-1, BMP-7, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, HB-EGF, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-13 Ra1, IL-18 BPa, IL-18 Rb, IL-18, IL-2 Ra, MMP-1, IL-2 Rb, IL-2 Rg, IL-21 R, MMP-2 , MMP-3, MMP-10, MMP-13).

Sensitivity: 3 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for TGFA ELISA Kit (ABIN625093)

Lim, Yoo, Kim, Hur, Lee, Hur, Lee, Lee, Park, Lee, Chang, Kim, Kang, Hong, Kim, Kim, Yoon, Nam, Yang, Kim, Cho, Won: "GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands." in: Molecular cancer therapeutics, Vol. 15, Issue 2, pp. 251-63, (2016) (PubMed).

Koob, Lim, Zabek, Massee: "Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 5, pp. 1133-40, (2015) (PubMed).

Briana, Liosi, Gourgiotis, Boutsikou, Marmarinos, Baka, Hassiakos, Malamitsi-Puchner: "Fetal concentrations of the growth factors TGF-α and TGF-β1 in relation to normal and restricted fetal growth at term." in: Cytokine, Vol. 60, Issue 1, pp. 157-61, (2013) (PubMed).

Miyabayashi, Ijichi, Mohri, Tada, Yamamoto, Asaoka, Ikenoue, Tateishi, Nakai, Isayama, Morishita, Omata, Moses, Koike: "Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals." in: Cancer research, Vol. 73, Issue 7, pp. 2221-34, (2013) (PubMed).

Drzymała-Czyż, Banasiewicz, Tubacka, Majewski, Biczysko, Kościński, Drews, Walkowiak: "Inulin supplementation in rat model of pouchitis." in: Acta biochimica Polonica, Vol. 58, Issue 3, pp. 381-4, (2012) (PubMed).

Franzke, Cobzaru, Triantafyllopoulou, Löffek, Horiuchi, Threadgill, Kurz, van Rooijen, Bruckner-Tuderman, Blobel: "Epidermal ADAM17 maintains the skin barrier by regulating EGFR ligand-dependent terminal keratinocyte differentiation." in: The Journal of experimental medicine, Vol. 209, Issue 6, pp. 1105-19, (2012) (PubMed).

Pinto, Di Fabio, Maiello, Pini, Latiano, Aschele, Garufi, Bochicchio, Rosati, Aprile, Giaquinta, Torri, Bardelli, Gion, Martoni: "Phase II study of panitumumab, oxaliplatin, 5-fluorouracil, and concurrent radiotherapy as preoperative treatment in high-risk locally advanced rectal cancer patients (StarPan/STAR-02 Study)." in: Annals of oncology : official journal of the European Society for Medical Oncology, Vol. 22, Issue 11, pp. 2424-30, (2011) (PubMed).

Acocella, Bertrand, Beytout, Durrande, Garcia Rodriguez, Kosmidis, Micoud, Rey, Rodriguez Zapata, Roux: "Comparison of three different regimens in the treatment of acute brucellosis: a multicenter multinational study." in: The Journal of antimicrobial chemotherapy, Vol. 23, Issue 3, pp. 433-9, (1989) (PubMed).

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF-alpha (TGFA Products)

Synonyms: TFGA ELISA Kit, LOC100009482 ELISA Kit, TGF alpha ELISA Kit, TGFA ELISA Kit, wa-1 ELISA Kit, wa1 ELISA Kit, RATTGFAA ELISA Kit, TGFAA ELISA Kit, NV15606 ELISA Kit, si:ch211-10e8.7 ELISA Kit, transforming growth factor alpha ELISA Kit, transforming growth factor alpha L homeolog ELISA Kit, transforming growth factor-alpha ELISA Kit, transforming growth factor, alpha ELISA Kit, TGFA ELISA Kit, TGF-A ELISA Kit, Tgfa ELISA Kit, tgfa.L ELISA Kit, LOC100121300 ELISA Kit, LOC100009150 ELISA Kit, tgfa ELISA Kit

Background: Gene Names: TGFA
Protein names: Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha (TGF-alpha) (EGF-like TGF) (ETGF) (TGF type 1)]

Gene ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway