Recommended Dilution for serum and plasma samples20 - 200 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 μL of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Restrictions
For Research Use only
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Fernández-Juárez, Villacorta Perez, Luño Fernández, Martinez-Martinez, Cachofeiro, Barrio Lucia, Tato Ribera, Mendez Abreu, Cordon, Oliva Dominguez, Praga Terente: "High levels of circulating TNFR1 increase the risk of all-cause mortality and progression of renal disease in type 2 diabetic nephropathy." in: Nephrology (Carlton, Vic.), Vol. 22, Issue 5, pp. 354-360, (2017) (PubMed).
Gao, Camous, Lu, Lim, Larbi, Ng: "Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies." in: Neurobiology of aging, Vol. 39, pp. 140-6, (2016) (PubMed).
Wu, Ding, Han, Arriens, Wei, Han, Pedroza, Jiang, Anolik, Petri, Sanz, Saxena, Mohan: "Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study." in: Journal of proteome research, Vol. 15, Issue 7, pp. 2102-14, (2016) (PubMed).
Sennikov, Alshevskaya, Shkaruba, Chumasova, Sizikov, Lopatnikova: "Expression of TNF? membrane-bound receptors in the peripheral blood mononuclear cells (PMBC) in rheumatoid arthritis patients." in: Cytokine, Vol. 73, Issue 2, pp. 288-94, (2015) (PubMed).
Bishayi, Bandyopadhyay, Majhi, Adhikary et al.: "Expression of CXCR1 (interleukin-8 receptor) in murine macrophages after staphylococcus aureus infection and its possible implication on intracellular survival correlating with cytokines and ..." in: Inflammation, Vol. 38, Issue 2, pp. 812-27, (2015) (PubMed).
Golikova, Lopatnikova, Kovalevskaya-Kucheryavenko, Nepomnyashih, Sennikov: "Levels of TNF, TNF autoantibodies and soluble TNF receptors in patients with bronchial asthma." in: The Journal of asthma : official journal of the Association for the Care of Asthma, Vol. 50, Issue 7, pp. 705-11, (2014) (PubMed).
Cuchacovich, Hagan, Khan, Richert, Espinoza: "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." in: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, (2011) (PubMed).
Issersohn: "[Radiologic examination of the colon with double contrast medium according to the Welin method]." in: Dapim refuiim. Folia medica, Vol. 24, Issue 1, pp. 27-31 (PubMed).