KIT Ligand ELISA Kit

Catalog No. ABIN625083

Product Details KIT Ligand ELISA Kit

Target: KIT Ligand (KITLG)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2-2000 pg/mL

Minimum Detection Limit: 2 pg/mL

Application: ELISA

Purpose: Human SCF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensitivity: 2 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 - 5 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: SCF (KITLG Products)

Synonyms: Xkl-1 ELISA Kit, Xsl ELISA Kit, Xsl-1 ELISA Kit, Xsl-2 ELISA Kit, steel ELISA Kit, KITLG ELISA Kit, SCF ELISA Kit, kitl ELISA Kit, kl-1 ELISA Kit, mgf ELISA Kit, scf ELISA Kit, FPH2 ELISA Kit, KL-1 ELISA Kit, Kitl ELISA Kit, MGF ELISA Kit, SF ELISA Kit, SHEP7 ELISA Kit, Clo ELISA Kit, Con ELISA Kit, Gb ELISA Kit, Kitlg ELISA Kit, Mgf ELISA Kit, SLF ELISA Kit, Sl ELISA Kit, blz ELISA Kit, contrasted ELISA Kit, 710-712 ELISA Kit, CSF ELISA Kit, KITL ELISA Kit, KIT ligand L homeolog ELISA Kit, KIT ligand ELISA Kit, kit ligand ELISA Kit, kitlg.L ELISA Kit, KITLG ELISA Kit, kitlg ELISA Kit, Kitl ELISA Kit, Kitlg ELISA Kit

Background: Gene Names: KITLG MGF SCF
Protein names: Kit ligand (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)]

Gene ID: 4254

UniProt: P21583

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway