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TNFSF14 ELISA Kit
TNFSF14
Reactivity: Human
Colorimetric
Sandwich ELISA
15-6000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details TNFSF14 ELISA Kit
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Target
See all TNFSF14 ELISA Kits
TNFSF14
(Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
Reactivity
All reactivities for TNFSF14 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
15-6000 pg/mL
Minimum Detection Limit
15 pg/mL
Application
ELISA
Purpose
Human LIGHT (TNFSF14) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity
15 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for TNFSF14
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Target
See all TNFSF14 ELISA Kits
TNFSF14
(Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
Alternative Name
LIGHT (TNFSF14 Products )
Synonyms
TNFSF14 ELISA Kit, CD258 ELISA Kit, HVEML ELISA Kit, LIGHT ELISA Kit, LTg ELISA Kit, TR2 ELISA Kit, HVEM-L ELISA Kit, Ly113 ELISA Kit, TNF superfamily member 14 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 14 ELISA Kit, TNFSF14 ELISA Kit, Tnfsf14 ELISA Kit
Background
Gene Names: TNFSF14 HVEML LIGHT UNQ391/PRO726 Protein names: Tumor necrosis factor ligand superfamily member 14 (Herpes virus entry mediator ligand) (HVEM-L) (Herpesvirus entry mediator ligand) (CD antigen CD258) [Cleaved into: Tumor necrosis factor ligand superfamily member 14, membrane form, Tumor necrosis factor ligand superfamily member 14, soluble form]
Gene ID
8740
UniProt
O43557
Pathways
Cancer Immune Checkpoints
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