RAB8B ELISA Kit
RAB8B
Reactivity: Human
Colorimetric
Sandwich ELISA
0.156 ng/mL - 10 ng/mL
Plasma, Serum, Tissue Homogenate
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- Target See all RAB8B ELISA Kits
- RAB8B (RAB8B, Member RAS Oncogene Family (RAB8B))
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 0.055 ng/mL
- Top Product
- Discover our top product RAB8B ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual - Sample Volume
- 100 µL
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all RAB8B ELISA Kits
- RAB8B (RAB8B, Member RAS Oncogene Family (RAB8B))
- Abstract
- RAB8B Products
- Synonyms
- 5930437D16 ELISA Kit, D330025I23Rik ELISA Kit, RAB8B, member RAS oncogene family ELISA Kit, RAB8B ELISA Kit, Rab8b ELISA Kit
- Background
- Alternative name: RAB-8b Protein, Ras-Related Protein Rab-8B
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Cell-Cell Junction Organization
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