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PLOD2 ELISA Kit

PLOD2 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5706925
  • Target See all PLOD2 ELISA Kits
    PLOD2 (Procollagen-Lysine 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0.061 ng/mL
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Pre-coated, ready to use 96-well strip plate,
    Standard (freeze dried),
    Standard Diluent,
    Detection Reagent A,
    Detection Reagent B,
    Assay Diluent A,
    Assay Diluent B,
    TMB substrate,
    Stop Solution,
    Wash Buffer(30 x concenthumane),
    Plate sealer for 96 wells,
    Instruction manual

    Sample Volume
    100 µL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all PLOD2 ELISA Kits
    PLOD2 (Procollagen-Lysine 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2))
    Alternative Name
    Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 2 (PLOD2) (PLOD2 Products)
    Synonyms
    D530025C14Rik ELISA Kit, LH2 ELISA Kit, Plod-2 ELISA Kit, TLH ELISA Kit, procollagen lysine, 2-oxoglutarate 5-dioxygenase 2 ELISA Kit, procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 ELISA Kit, Plod2 ELISA Kit, PLOD2 ELISA Kit
    Background
    Alternative name: TLH, LH2, Telopeptide Lysyl Hydroxylase, Lysyl hydroxylase 2
    Pathways
    SARS-CoV-2 Protein Interactome
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