MYOD1 ELISA Kit

Catalog No. ABIN5706920

Product Details MYOD1 ELISA Kit

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.1 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.1 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 29 pg/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: Myogenic Differentiation (MyoD) (MYOD1 Products)

Synonyms: MYF3 ELISA Kit, MYOD ELISA Kit, PUM ELISA Kit, bHLHc1 ELISA Kit, MyoD ELISA Kit, XMyoD ELISA Kit, XtmyoD ELISA Kit, mf25 ELISA Kit, myf3 ELISA Kit, pum ELISA Kit, MUF3 ELISA Kit, AI503393 ELISA Kit, Myod-1 ELISA Kit, etID309723.25 ELISA Kit, myod ELISA Kit, wu:fb57a01 ELISA Kit, zgc:136744 ELISA Kit, MYOD1 ELISA Kit, myod1 ELISA Kit, mf1 ELISA Kit, myod1-a ELISA Kit, myod1-b ELISA Kit, myogenic differentiation 1 ELISA Kit, MYOD protein ELISA Kit, myogenic factor MyoD1 ELISA Kit, myogenic differentiation 1 S homeolog ELISA Kit, myogenic differentiation 1 L homeolog ELISA Kit, MYOD1 ELISA Kit, myod1 ELISA Kit, Myod1 ELISA Kit, myod ELISA Kit, myod1.S ELISA Kit, myod1.L ELISA Kit

Background: Alternative name: MYOD1, PUM, MYF3, bHLHc1, Myoblast Determination Protein 1, Myogenic Factor 3, Class C basic helix-loop-helix protein 1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development