GRIN2B ELISA Kit

Catalog No. ABIN5706918

Product Details GRIN2B ELISA Kit

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.133 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B) (GRIN2B Products)

Synonyms: NMDAR2B ELISA Kit, GluN2B ELISA Kit, MRD6 ELISA Kit, NR2B ELISA Kit, hNR3 ELISA Kit, nmdar2b ELISA Kit, nr2b ELISA Kit, AW490526 ELISA Kit, Nmdar2b ELISA Kit, glutamate ionotropic receptor NMDA type subunit 2B ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 2B L homeolog ELISA Kit, glutamate receptor, ionotropic, NMDA2B (epsilon 2) ELISA Kit, GRIN2B ELISA Kit, Grin2b ELISA Kit, grin2b.L ELISA Kit

Background: Alternative name: NMDAR2B, NR2B, hNR3, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity