STUB1 ELISA Kit

Catalog No. ABIN5706909

Product Details STUB1 ELISA Kit

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.051 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for STUB1

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Abstract: STUB1 Products

Synonyms: CHIP ELISA Kit, HSPABP2 ELISA Kit, NY-CO-7 ELISA Kit, SDCCAG7 ELISA Kit, UBOX1 ELISA Kit, 0610033N24Rik ELISA Kit, 2210017D18Rik ELISA Kit, 2310040B03Rik ELISA Kit, AW046544 ELISA Kit, Chip ELISA Kit, wu:fc22f04 ELISA Kit, zgc:56076 ELISA Kit, STUB1 ELISA Kit, chip ELISA Kit, ATCHIP ELISA Kit, CARBOXYL TERMINUS OF HSC70-INTERACTING PROTEIN ELISA Kit, carboxyl terminus of HSC70-interacting protein ELISA Kit, STIP1 homology and U-box containing protein 1 ELISA Kit, STIP1 homology and U-Box containing protein 1 ELISA Kit, STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase L homeolog ELISA Kit, STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase ELISA Kit, STIP1 homology and U box-containing protein 1 ELISA Kit, carboxyl terminus of HSC70-interacting protein ELISA Kit, STUB1 ELISA Kit, Stub1 ELISA Kit, stub1 ELISA Kit, stub1.L ELISA Kit, LOC100282870 ELISA Kit, CHIP ELISA Kit

Background: Alternative name: CHIP, HSPABP2, NY-CO-7, SDCCAG7, UBOX1, C Terminus Of HSC70-Interacting Protein, CLL-associated antigen KW-8, E3 ubiquitin-protein ligase CHIP

Pathways: Regulation of Hormone Metabolic Process, Response to Water Deprivation