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Myosin X ELISA Kit

MYO10 Reactivity: Human Colorimetric Competition ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5706904
  • Target See all Myosin X (MYO10) products
    Myosin X (MYO10)
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Purpose
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MYO10 in human serum, plasma, tissue homogenates
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0.114 ng/mL
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Pre-coated, ready to use 96-well strip plate,
    Standard (freeze dried),
    Standard Diluent,
    Detection Reagent A,
    Detection Reagent B,
    Assay Diluent A,
    Assay Diluent B,
    TMB substrate,
    Stop Solution,
    Wash Buffer(30 x concenthumane),
    Plate sealer for 96 wells,
    Instruction manual

    Sample Volume
    50 µL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C
    3. Aspirate and wash 3 times
    4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    5. Aspirate and wash 5 times
    6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    7. Add 50µL Stop Solution. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all Myosin X (MYO10) products
    Myosin X (MYO10)
    Abstract
    MYO10 Products
    Synonyms
    AW048724 ELISA Kit, D15Ertd600e ELISA Kit, mKIAA0799 ELISA Kit, MYO10 ELISA Kit, myosin-x ELISA Kit, myox ELISA Kit, myosin X ELISA Kit, myosin 10 ELISA Kit, myosin X-like 3 ELISA Kit, unconventional myosin-IXb ELISA Kit, myosin X-like ELISA Kit, unconventional myosin-X ELISA Kit, MYO10 ELISA Kit, Myo10 ELISA Kit, myo10 ELISA Kit, myo10l3 ELISA Kit, LOC5576295 ELISA Kit, LOC100548805 ELISA Kit, LOC100641156 ELISA Kit
    Background
    Alternative name: Unconventional Myosin-X, Unconventional Myosin-10
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