Myosin X ELISA Kit
MYO10
Reactivity: Human
Colorimetric
Competition ELISA
0.312 ng/mL - 20 ng/mL
Plasma, Serum, Tissue Homogenate
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- Target See all Myosin X (MYO10) products
- Myosin X (MYO10)
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.312 ng/mL
- Application
- ELISA
- Purpose
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MYO10 in human serum, plasma, tissue homogenates
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 0.114 ng/mL
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual - Sample Volume
- 50 µL
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5. Aspirate and wash 5 times
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50µL Stop Solution. Read at 450 nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
-
- Target See all Myosin X (MYO10) products
- Myosin X (MYO10)
- Abstract
- MYO10 Products
- Background
- Alternative name: Unconventional Myosin-X, Unconventional Myosin-10
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