Metabotropic Glutamate Receptor 1 ELISA Kit

Catalog No. ABIN5706903

Product Details Metabotropic Glutamate Receptor 1 ELISA Kit

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.147 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for Metabotropic Glutamate Receptor 1

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Abstract: GRM1 Products

Synonyms: fj39d05 ELISA Kit, wu:fj39d05 ELISA Kit, si:ch211-51a19.1 ELISA Kit, Glura ELISA Kit, mGlutR ELISA Kit, GB18621 ELISA Kit, GPRC1A ELISA Kit, MGLU1 ELISA Kit, MGLUR1 ELISA Kit, SCAR13 ELISA Kit, 4930455H15Rik ELISA Kit, ENSMUSG00000075319 ELISA Kit, Gm10828 ELISA Kit, Gprc1a ELISA Kit, mGluR1 ELISA Kit, nmf373 ELISA Kit, rcw ELISA Kit, wobl ELISA Kit, glutamate metabotropic receptor 1 ELISA Kit, glutamate receptor, metabotropic 1a ELISA Kit, metabotropic glutamate receptor 1 ELISA Kit, glutamate receptor, metabotropic 1 ELISA Kit, GRM1 ELISA Kit, grm1a ELISA Kit, mGlutR1 ELISA Kit, Grm1 ELISA Kit

Background: Alternative name: GPRC1A, GRM1A, MGLUR1, MGLUR1A, mGluR1