ATP1B3 ELISA Kit
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- Target See all ATP1B3 ELISA Kits
- ATP1B3 (ATPase, Na+/K+ Transporting, beta 3 Polypeptide (ATP1B3))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 0.068 ng/mL
- Top Product
- Discover our top product ATP1B3 ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual - Sample Volume
- 100 µL
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all ATP1B3 ELISA Kits
- ATP1B3 (ATPase, Na+/K+ Transporting, beta 3 Polypeptide (ATP1B3))
- Alternative Name
- ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) (ATP1B3 Products)
- Synonyms
- ATPB-3 ELISA Kit, CD298 ELISA Kit, AA409958 ELISA Kit, AI664000 ELISA Kit, AW212096 ELISA Kit, NKAB3S ELISA Kit, atp1b3 ELISA Kit, cb714 ELISA Kit, fb13c07 ELISA Kit, wu:fb13c07 ELISA Kit, cb713 ELISA Kit, nakatpb ELISA Kit, wu:fa14a07 ELISA Kit, ATPase Na+/K+ transporting subunit beta 3 ELISA Kit, ATPase, Na+/K+ transporting, beta 3 polypeptide ELISA Kit, ATPase Na+/K+ transporting subunit beta 3 S homeolog ELISA Kit, ATPase, Na+/K+ transporting, beta 3b polypeptide ELISA Kit, ATPase, Na+/K+ transporting, beta 3a polypeptide ELISA Kit, ATP1B3 ELISA Kit, Atp1b3 ELISA Kit, atp1b3.S ELISA Kit, atp1b3b ELISA Kit, atp1b3a ELISA Kit
- Background
- Alternative name: CD298, ATP1-B3, ATPB-3, Sodium/Potassium-Transporting ATPase Subunit beta-3
- Pathways
- Thyroid Hormone Synthesis
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