CFP ELISA Kit

Catalog No. ABIN5692201

Product Details CFP ELISA Kit

Target: CFP (Complement Factor P (CFP))

Binding Specificity: AA 28-469

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Properdin. 96wells/kit, with removable strips.

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO, Immunogen sequence: D28-L469

Sensitivity: < 10 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 0.5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for CFP

Target: CFP (Complement Factor P (CFP))

Alternative Name: CFP (CFP Products)

Synonyms: BFD ELISA Kit, PFC ELISA Kit, PFD ELISA Kit, PROPERDIN ELISA Kit, BCFG ELISA Kit, Pfc ELISA Kit, Properdin ELISA Kit, complement factor properdin ELISA Kit, CFP ELISA Kit, Cfp ELISA Kit

Background:

Synonyms: Properdin, Complement factor P, CFP, PFC

Background: Properdin or Factor P is the only known positive regulator of complement activation that stabilizes thealternative pathway convertases. This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Cellular Localisation: Secreted.

UniProt: P27918

Pathways: Complement System