IKK alpha FLISA Kit

Catalog No. ABIN5691968

Product Details IKK alpha FLISA Kit

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Binding Specificity: pSer176, pSer177

Reactivity: Human, Mouse, Rat

Detection Method: Fluorometric

Method Type: Cell ELISA

Application: ELISA

Purpose: The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.

Brand: CytoFluor™

Sample Type: Cell Culture Cells

Analytical Method: Qualitative

Characteristics: Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma

Components: 2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual

Application Details

Application Notes: Detection Range: At least 5000 cells/well

Assay Time: 4.5 h

Plate: Uncoated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C/6 Months

Expiry Date: 6 months

Target Details

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: IKK-alpha (CHUK Products)

Synonyms: ikk FLISA Kit, ikk1 FLISA Kit, ikka FLISA Kit, ikkalpha FLISA Kit, CHUK FLISA Kit, IKBKA FLISA Kit, IKK-alpha FLISA Kit, IKK1 FLISA Kit, IKKA FLISA Kit, NFKBIKA FLISA Kit, TCF16 FLISA Kit, AI256658 FLISA Kit, Chuk1 FLISA Kit, Fbx24 FLISA Kit, Fbxo24 FLISA Kit, Ikka FLISA Kit, zgc:56539 FLISA Kit, conserved helix-loop-helix ubiquitous kinase S homeolog FLISA Kit, conserved helix-loop-helix ubiquitous kinase FLISA Kit, chuk.S FLISA Kit, CHUK FLISA Kit, Chuk FLISA Kit, chuk FLISA Kit

UniProt: O15111, O14920

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins