IKK alpha FLISA Kit
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- Target See all IKK alpha (CHUK) FLISA Kits
- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
- Binding Specificity
- pSer176, pSer177
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Reactivity
- Human, Mouse, Rat
- Detection Method
- Fluorometric
- Method Type
- Cell ELISA
- Application
- ELISA
- Purpose
- The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.
- Brand
- CytoFluor™
- Sample Type
- Cell Culture Cells
- Analytical Method
- Qualitative
- Characteristics
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Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma - Components
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2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual - Top Product
- Discover our top product CHUK ELISA Kit
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conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) ELISA Kit
CHUK Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) ELISA KitHigh Sensitivity CHUK Reactivity: Human Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Lysate, Tissue Homogenate
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- Application Notes
- Detection Range: At least 5000 cells/well
- Assay Time
- 4.5 h
- Plate
- Uncoated
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- 4°C/6 Months
- Expiry Date
- 6 months
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- Target See all IKK alpha (CHUK) FLISA Kits
- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
- Alternative Name
- IKK-alpha (CHUK Products)
- UniProt
- O15111, O14920
- Pathways
- PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins
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