AKT1 FLISA Kit

Catalog No. ABIN5691895

Product Details AKT1 FLISA Kit

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Binding Specificity: pSer473

Reactivity: Human, Mouse, Rat

Detection Method: Fluorometric

Method Type: Cell ELISA

Application: ELISA

Purpose: The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.

Brand: CytoFluor™

Sample Type: Cell Culture Cells

Analytical Method: Qualitative

Characteristics: Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma

Components: 2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual

Application Details

Application Notes: Detection Range: At least 5000 cells/well

Assay Time: 4.5 h

Plate: Uncoated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C/6 Months

Expiry Date: 6 months

Target Details

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: Akt1 (AKT1 Products)

Synonyms: AKT FLISA Kit, CWS6 FLISA Kit, PKB FLISA Kit, PKB-ALPHA FLISA Kit, PRKBA FLISA Kit, RAC FLISA Kit, RAC-ALPHA FLISA Kit, Akt FLISA Kit, Rac FLISA Kit, PKB/Akt FLISA Kit, PKBalpha FLISA Kit, AKT/PKB FLISA Kit, AKT1 FLISA Kit, Akt/PKB FLISA Kit, Akt1 FLISA Kit, CG4006 FLISA Kit, D-Akt FLISA Kit, DAKT1 FLISA Kit, DAKT1/PKB FLISA Kit, DAkt FLISA Kit, DAkt1 FLISA Kit, DPKB FLISA Kit, DRAC-PK FLISA Kit, DRAC-PK66 FLISA Kit, DRAC-PK85 FLISA Kit, Dakt FLISA Kit, Dakt1 FLISA Kit, Dmel\\CG4006 FLISA Kit, Dpkb FLISA Kit, PKB/AKT FLISA Kit, PKB/dAKT FLISA Kit, RacPK FLISA Kit, akt FLISA Kit, akt1 FLISA Kit, dAKT FLISA Kit, dAKT/dPKB FLISA Kit, dAKT1 FLISA Kit, dAkt FLISA Kit, dAkt/PKB FLISA Kit, dAkt1 FLISA Kit, dPKB FLISA Kit, dakt FLISA Kit, dakt1 FLISA Kit, l(3)04226 FLISA Kit, l(3)89Bq FLISA Kit, p-Akt FLISA Kit, pAkt FLISA Kit, ACT-5 FLISA Kit, akt-1 FLISA Kit, pkb FLISA Kit, v-akt FLISA Kit, v-akt1 FLISA Kit, xAct FLISA Kit, ATAKT1 FLISA Kit, F18A8.2 FLISA Kit, F18A8_2 FLISA Kit, K+ transporter 1 FLISA Kit, POTASSIUM TRANSPORTER FLISA Kit, AKT serine/threonine kinase 1 FLISA Kit, thymoma viral proto-oncogene 1 FLISA Kit, CG4006 gene product from transcript CG4006-RE FLISA Kit, actin beta FLISA Kit, v-akt murine thymoma viral oncogene homolog 1 S homeolog FLISA Kit, v-akt murine thymoma viral oncogene homolog 1 FLISA Kit, K+ transporter 1 FLISA Kit, Serine/threonine-protein kinase akt-1 FLISA Kit, AKT1 FLISA Kit, Akt1 FLISA Kit, ACTB FLISA Kit, akt1.S FLISA Kit, akt1 FLISA Kit, KT1 FLISA Kit, akt-1 FLISA Kit

Gene ID: 207

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect