AIF ELISA Kit
AIFM1
Reactivity: Sheep
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all AIF (AIFM1) ELISA Kits
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AIF (AIFM1)
(Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
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Reactivity
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Sheep
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Ovine (sheep) AIF ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificity
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This ELISA antibody pair recognizes Ovine (sheep) AIF.
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Storage
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4 °C
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Expiry Date
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6 months
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Target
See all AIF (AIFM1) ELISA Kits
-
AIF (AIFM1)
(Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
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Alternative Name
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AIF (AIFM1 Products)
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Synonyms
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AIF ELISA Kit, CMTX4 ELISA Kit, COWCK ELISA Kit, COXPD6 ELISA Kit, PDCD8 ELISA Kit, CG7263 ELISA Kit, DmAIF ELISA Kit, Dmel\\CG7263 ELISA Kit, GB16024 ELISA Kit, DDBDRAFT_0187853 ELISA Kit, DDBDRAFT_0191137 ELISA Kit, DDB_0187853 ELISA Kit, DDB_0191137 ELISA Kit, aif ELISA Kit, pdcd8 ELISA Kit, AIFM1 ELISA Kit, PCD8 ELISA Kit, AIFsh2 ELISA Kit, Hq ELISA Kit, Pdcd8 ELISA Kit, mAIF ELISA Kit, Aif ELISA Kit, zgc:91994 ELISA Kit, apoptosis inducing factor mitochondria associated 1 ELISA Kit, allograft inflammatory factor 1 ELISA Kit, Apoptosis inducing factor ELISA Kit, apoptosis-inducing factor 1, mitochondrial ELISA Kit, apoptosis inducing factor ELISA Kit, apoptosis inducing factor, mitochondria associated 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated, 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated 1 ELISA Kit, AIFM1 ELISA Kit, AIF1 ELISA Kit, AIF ELISA Kit, LOC412212 ELISA Kit, aif ELISA Kit, aifm1 ELISA Kit, Aifm1 ELISA Kit
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Pathways
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Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect
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