UFM1 ELISA Kit

Catalog No. ABIN5690967

Product Details UFM1 ELISA Kit

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human UFM1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human UFM1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for UFM1

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Alternative Name: UFM1 (UFM1 Products)

Synonyms: T32E8.4 ELISA Kit, T32E8_4 ELISA Kit, BM-002 ELISA Kit, C13orf20 ELISA Kit, bA131P10.1 ELISA Kit, 1810045K17Rik ELISA Kit, AI132708 ELISA Kit, AI463323 ELISA Kit, ENSMUSG00000074598 ELISA Kit, Gm10726 ELISA Kit, RGD1304890 ELISA Kit, UBFM1 ELISA Kit, ubfm1 ELISA Kit, wu:fa10f05 ELISA Kit, wu:fb72g01 ELISA Kit, zgc:55335 ELISA Kit, ubiquitin fold modifier 1 ELISA Kit, ubiquitin-fold modifier 1 ELISA Kit, ubiquitin-fold modifier ELISA Kit, ubiquitin-fold modifier 1 L homeolog ELISA Kit, UFM1 ELISA Kit, ufm1 ELISA Kit, CCP2 ELISA Kit, Ufm1 ELISA Kit, ufm1.L ELISA Kit, PR46a ELISA Kit

Gene ID: 51569

UniProt: P61960