Tryptophan Hydroxylase 1 ELISA Kit

Catalog No. ABIN5690961

Product Details Tryptophan Hydroxylase 1 ELISA Kit

Target: Tryptophan Hydroxylase 1 (TPH1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human TPH-1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human TPH-1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Alternative Name: TPH-1 (TPH1 Products)

Synonyms: TPRH ELISA Kit, TRPH ELISA Kit, tph ELISA Kit, tph1 ELISA Kit, tphD1 ELISA Kit, tph2 ELISA Kit, tph1l ELISA Kit, tphD2 ELISA Kit, Tph ELISA Kit, TPH ELISA Kit, SERGEF ELISA Kit, LOC100229598 ELISA Kit, tryptophan hydroxylase 1 ELISA Kit, tryptophan hydroxylase 1 (tryptophan 5-monooxygenase) a ELISA Kit, TryPtophan Hydroxylase ELISA Kit, tryptophan hydroxylase 1b ELISA Kit, tryptophan hydroxylase 1 L homeolog ELISA Kit, tryptophan 5-hydroxylase 1 ELISA Kit, TPH1 ELISA Kit, tph1a ELISA Kit, tph-1 ELISA Kit, tph1b ELISA Kit, Tph1 ELISA Kit, tph1.L ELISA Kit, LOAG_02149 ELISA Kit, Tsp_05203 ELISA Kit

Gene ID: 7166

UniProt: P17752

Pathways: Feeding Behaviour