TCF7L1 ELISA Kit

Catalog No. ABIN5690960

Product Details TCF7L1 ELISA Kit

Target: TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human TCF7L1/TCF-3 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human TCF7L1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for TCF7L1

Target: TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))

Alternative Name: TCF7L1 (TCF-3 (TCF7L1 Products)

Synonyms: TCF-3 ELISA Kit, TCF3 ELISA Kit, Tcf-3 ELISA Kit, Tcf3 ELISA Kit, bHLHb21 ELISA Kit, tcf3c ELISA Kit, tcf3d ELISA Kit, xtcf3 ELISA Kit, XTCF-3 ELISA Kit, tcf7l1 ELISA Kit, tcf3b ELISA Kit, TCF-3-A ELISA Kit, tcf-3 ELISA Kit, tcf3 ELISA Kit, tcf3a ELISA Kit, zTcf-3 ELISA Kit, transcription factor 7 like 1 ELISA Kit, transcription factor 7 like 1 (T cell specific, HMG box) ELISA Kit, transcription factor 7 like 1 L homeolog ELISA Kit, transcription factor 7 like 1 S homeolog ELISA Kit, transcription factor 7 like 1b ELISA Kit, transcription factor 7 like 1a ELISA Kit, TCF7L1 ELISA Kit, Tcf7l1 ELISA Kit, tcf7l1.L ELISA Kit, tcf7l1.S ELISA Kit, tcf7l1b ELISA Kit, tcf7l1a ELISA Kit

Gene ID: 83439

UniProt: Q9HCS4

Pathways: WNT Signaling, Stem Cell Maintenance