SOX21 ELISA Kit

Catalog No. ABIN5690956

Product Details SOX21 ELISA Kit

Target: SOX21 (SRY (Sex Determining Region Y)-Box 21 (SOX21))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human SOX21/SOX-A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human SOX21.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for SOX21

Target: SOX21 (SRY (Sex Determining Region Y)-Box 21 (SOX21))

Alternative Name: SOX21 (SOX-A (SOX21 Products)

Synonyms: SOX25 ELISA Kit, Sox25 ELISA Kit, CG7345 ELISA Kit, Dmel\\CG7345 ELISA Kit, SOXB2.3 ELISA Kit, Sox B2-3 ELISA Kit, Sox21 ELISA Kit, SoxB2.3 ELISA Kit, sox25 ELISA Kit, SRY-box ELISA Kit, sox21 ELISA Kit, sox30 ELISA Kit, zgc:110678 ELISA Kit, CH31 ELISA Kit, SRY-box 21 ELISA Kit, SRY (sex determining region Y)-box 21 ELISA Kit, SRY-box 21 S homeolog ELISA Kit, CG7345 gene product from transcript CG7345-RA ELISA Kit, SRY box 21 ELISA Kit, SRY (sex determining region Y)-box 21a ELISA Kit, SRY (sex determining region Y)-box 21b ELISA Kit, SOX21 ELISA Kit, Sox21 ELISA Kit, sox21.S ELISA Kit, Sox21a ELISA Kit, sox21 ELISA Kit, sox21a ELISA Kit, sox21b ELISA Kit

Gene ID: 11166

UniProt: Q9Y651