SLPI ELISA Kit

Catalog No. ABIN5690952

Product Details SLPI ELISA Kit

Target: SLPI (Secretory Leukocyte Peptidase Inhibitor (SLPI))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human SLPI ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human SLPI.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for SLPI

Target: SLPI (Secretory Leukocyte Peptidase Inhibitor (SLPI))

Alternative Name: SLPI (SLPI Products)

Synonyms: ALK1 ELISA Kit, ALP ELISA Kit, BLPI ELISA Kit, HUSI ELISA Kit, HUSI-I ELISA Kit, MPI ELISA Kit, WAP4 ELISA Kit, WFDC4 ELISA Kit, MATN4 ELISA Kit, SLPI ELISA Kit, ckpi ELISA Kit, secretory leukocyte peptidase inhibitor ELISA Kit, antileukoproteinase ELISA Kit, SLPI ELISA Kit, Slpi ELISA Kit, LOC102174044 ELISA Kit

Gene ID: 6590

UniProt: P03973