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PIK3R3 ELISA Kit

PIK3R3 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN5690933
  • Target See all PIK3R3 ELISA Kits
    PIK3R3 (Phosphatidylinositol 3-kinase regulatory subunit gamma (PIK3R3))
    Reactivity
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human PI3-Kinase p55-gamma ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human PI3-Kinase p55-gamma.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Target See all PIK3R3 ELISA Kits
    PIK3R3 (Phosphatidylinositol 3-kinase regulatory subunit gamma (PIK3R3))
    Alternative Name
    PI3-Kinase p55-gamma (PIK3R3 Products)
    Synonyms
    AA414954 ELISA Kit, p55pik ELISA Kit, p55 ELISA Kit, p55-GAMMA ELISA Kit, pik3r3 ELISA Kit, zgc:55564 ELISA Kit, zgc:85710 ELISA Kit, phosphoinositide-3-kinase regulatory subunit 3 ELISA Kit, phosphoinositide-3-kinase, regulatory subunit 3 (gamma) ELISA Kit, phosphoinositide-3-kinase, regulatory subunit 3b (gamma) ELISA Kit, PIK3R3 ELISA Kit, Pik3r3 ELISA Kit, pik3r3b ELISA Kit
    Gene ID
    8503
    UniProt
    Q92569
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