Peroxiredoxin 5 ELISA Kit

Catalog No. ABIN5690930

Product Details Peroxiredoxin 5 ELISA Kit

Target: Peroxiredoxin 5 (PRDX5)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Peroxiredoxin-5 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Peroxiredoxin-5.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for Peroxiredoxin 5

Target: Peroxiredoxin 5 (PRDX5)

Alternative Name: Peroxiredoxin-5 (PRDX5 Products)

Synonyms: acr1 ELISA Kit, aoeb166 ELISA Kit, pmp20 ELISA Kit, prx-5 ELISA Kit, prx5 ELISA Kit, prxv ELISA Kit, sbbi10 ELISA Kit, GB16965 ELISA Kit, zgc:112318 ELISA Kit, PRDX5 ELISA Kit, CG 32920 ELISA Kit, CG 7217 ELISA Kit, CG32920 ELISA Kit, CG7217 ELISA Kit, Dmel\\CG7217 ELISA Kit, dPrx5 ELISA Kit, dprx5 ELISA Kit, ACR1 ELISA Kit, AOEB166 ELISA Kit, B166 ELISA Kit, PLP ELISA Kit, PMP20 ELISA Kit, PRDX6 ELISA Kit, PRXV ELISA Kit, prx-V ELISA Kit, AOPP ELISA Kit, Pmp20 ELISA Kit, Prdx6 ELISA Kit, PrxV ELISA Kit, Aoeb166 ELISA Kit, Prx V ELISA Kit, peroxiredoxin 5 L homeolog ELISA Kit, peroxiredoxin-5, mitochondrial ELISA Kit, peroxiredoxin 5 ELISA Kit, Peroxiredoxin 5 ELISA Kit, prdx5.L ELISA Kit, LOC552429 ELISA Kit, prdx5 ELISA Kit, PRDX5 ELISA Kit, Prx5 ELISA Kit, Prdx5 ELISA Kit

Gene ID: 25824

UniProt: P30044

Pathways: Cell RedoxHomeostasis