Parkin ELISA Kit

Catalog No. ABIN5690927

Product Details Parkin ELISA Kit

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Parkin ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Parkin.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Alternative Name: Parkin (PARK2 Products)

Synonyms: CG10523 ELISA Kit, Dmel\\CG10523 ELISA Kit, Dpark ELISA Kit, SD01679 ELISA Kit, dpk ELISA Kit, AR-JP ELISA Kit, LPRS2 ELISA Kit, PDJ ELISA Kit, PRKN ELISA Kit, Park ELISA Kit, Prkn ELISA Kit, si:ch211-123f21.1 ELISA Kit, zgc:112390 ELISA Kit, pdr-1 ELISA Kit, PARK2 ELISA Kit, parkin ELISA Kit, parkin RBR E3 ubiquitin protein ligase ELISA Kit, Parkinson disease (autosomal recessive, juvenile) 2, parkin ELISA Kit, park ELISA Kit, PRKN ELISA Kit, Prkn ELISA Kit, prkn ELISA Kit, CpipJ_CPIJ014867 ELISA Kit, Park2 ELISA Kit

Gene ID: 5071

UniProt: O60260

Pathways: Autophagy, Ubiquitin Proteasome Pathway