MAPK12 ELISA Kit

Catalog No. ABIN5690925

Product Details MAPK12 ELISA Kit

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human p38 gamma/MAPK12/ERK-6 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human p38 gamma.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for MAPK12

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Alternative Name: p38 gamma (MAPK12/ERK-6) (MAPK12 Products)

Synonyms: ATMPK12 ELISA Kit, MAPK12 ELISA Kit, T3F17.28 ELISA Kit, mitogen-activated protein kinase 12 ELISA Kit, MGC160082 ELISA Kit, si:dkey-14d8.5 ELISA Kit, erk6 ELISA Kit, etID309866.18 ELISA Kit, mapk12 ELISA Kit, sapk3 ELISA Kit, wu:fa05c12 ELISA Kit, zgc:101695 ELISA Kit, ERK3 ELISA Kit, ERK6 ELISA Kit, P38GAMMA ELISA Kit, PRKM12 ELISA Kit, SAPK-3 ELISA Kit, SAPK3 ELISA Kit, AW123708 ELISA Kit, Erk6 ELISA Kit, P38gamma ELISA Kit, Prkm12 ELISA Kit, Sapk3 ELISA Kit, mitogen-activated protein kinase 12 ELISA Kit, mitogen-activated protein kinase 12b ELISA Kit, mitogen-activated protein kinase 12a ELISA Kit, MPK12 ELISA Kit, MAPK12 ELISA Kit, mapk12b ELISA Kit, mapk12a ELISA Kit, Mapk12 ELISA Kit

Gene ID: 6300

UniProt: P53778

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteins