Neurogenin 3 ELISA Kit

Catalog No. ABIN5690917

Product Details Neurogenin 3 ELISA Kit

Target: Neurogenin 3 (NEUROG3)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Neurogenin-3 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Neurogenin-3.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for Neurogenin 3

Target: Neurogenin 3 (NEUROG3)

Alternative Name: Neurogenin-3 (NEUROG3 Products)

Synonyms: NEUROG3 ELISA Kit, ngn3 ELISA Kit, atoh5 ELISA Kit, ngn-3 ELISA Kit, bhlha7 ELISA Kit, math4b ELISA Kit, neurog3 ELISA Kit, Ngn3 ELISA Kit, Atoh5 ELISA Kit, Math4B ELISA Kit, NGN-3 ELISA Kit, bHLHa7 ELISA Kit, Relax ELISA Kit, atoh4 ELISA Kit, ngn2 ELISA Kit, ngn3a ELISA Kit, neurogenin 3 ELISA Kit, neurogenin 3 S homeolog ELISA Kit, neurogenin-3 ELISA Kit, NEUROG3 ELISA Kit, neurog3 ELISA Kit, neurog3.S ELISA Kit, Tsp_02556 ELISA Kit, Neurog3 ELISA Kit

Gene ID: 50674

UniProt: Q9Y4Z2