NEDD8 ELISA Kit

Catalog No. ABIN5690916

Product Details NEDD8 ELISA Kit

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human NEDD8 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human NEDD8.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: NEDD8 (NEDD8 Products)

Synonyms: NEDD-8 ELISA Kit, Rub1 ELISA Kit, CG10679 ELISA Kit, Dmel\\CG10679 ELISA Kit, NEDD8 ELISA Kit, dNEDD8 ELISA Kit, nedd8 ELISA Kit, ubiquitin ELISA Kit, si:zc14a17.5 ELISA Kit, GB17785 ELISA Kit, DDBDRAFT_0218116 ELISA Kit, DDBDRAFT_0238041 ELISA Kit, DDB_0218116 ELISA Kit, DDB_0238041 ELISA Kit, nedd8.S ELISA Kit, neural precursor cell expressed, developmentally down-regulated 8 ELISA Kit, neural precursor cell expressed, developmentally down-regulated gene 8 ELISA Kit, CG10679 gene product from transcript CG10679-RB ELISA Kit, Nedd8 ELISA Kit, ubiquitin-like protein Nedd8 ELISA Kit, neural precursor cell expressed, developmentally down-regulated 8 L homeolog ELISA Kit, NEDD8 ELISA Kit, Nedd8 ELISA Kit, nedd8 ELISA Kit, nedd8.L ELISA Kit

Gene ID: 4738

UniProt: Q15843

Pathways: Ubiquitin Proteasome Pathway