Myogenin ELISA Kit
MYOG
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all Myogenin (MYOG) ELISA Kits
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Myogenin (MYOG)
(Myogenin (Myogenic Factor 4) (MYOG))
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Reactivity
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Human
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Human Myogenin ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificity
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This ELISA antibody pair recognizes Human Myogenin.
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Storage
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4 °C
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Expiry Date
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6 months
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Target
See all Myogenin (MYOG) ELISA Kits
-
Myogenin (MYOG)
(Myogenin (Myogenic Factor 4) (MYOG))
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Alternative Name
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Myogenin (MYOG Products)
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Synonyms
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cb553 ELISA Kit, zgc:100763 ELISA Kit, LOC446037 ELISA Kit, myf4 ELISA Kit, Xmyog ELISA Kit, myogenin ELISA Kit, LOC100136471 ELISA Kit, LOC100224062 ELISA Kit, LOC100303673 ELISA Kit, MYF4 ELISA Kit, bHLHc3 ELISA Kit, myo ELISA Kit, myf-4 ELISA Kit, myogenin ELISA Kit, myogenin (myogenic factor 4) ELISA Kit, MYOG ELISA Kit, myog ELISA Kit, LOC100303673 ELISA Kit, Myog ELISA Kit
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Gene ID
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4656
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UniProt
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P15173
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Pathways
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Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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