Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

JNK2 ELISA Kit

MAPK9 Reactivity: Human Isoform alpha-1 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN5690901
  • Target See all JNK2 (MAPK9) ELISA Kits
    JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))
    Binding Specificity
    Isoform alpha-1
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human JNK2/MAPK9 isoform alpha-1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human JNK2.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling MAPK9 ELISA Kit
    Top Product
    Discover our top product MAPK9 ELISA Kit
  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Target See all JNK2 (MAPK9) ELISA Kits
    JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))
    Alternative Name
    JNK2 (MAPK9 (MAPK9 Products)
    Synonyms
    MAPK9 ELISA Kit, JNK-55 ELISA Kit, JNK2 ELISA Kit, JNK2A ELISA Kit, JNK2ALPHA ELISA Kit, JNK2B ELISA Kit, JNK2BETA ELISA Kit, PRKM9 ELISA Kit, SAPK ELISA Kit, SAPK1a ELISA Kit, p54a ELISA Kit, p54aSAPK ELISA Kit, AI851083 ELISA Kit, Prkm9 ELISA Kit, JNK2-ALPHA1 ELISA Kit, MAP kinase 9 ELISA Kit, mitogen-activated protein kinase 9 ELISA Kit, MAP kinase 9 ELISA Kit, MAPK9 ELISA Kit, Mapk9 ELISA Kit, MPK9 ELISA Kit
    Pathways
    MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins
You are here:
Support