JNK2 ELISA Kit
MAPK9
Reactivity: Human
Isoform alpha-1
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all JNK2 (MAPK9) ELISA Kits
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JNK2 (MAPK9)
(Mitogen-Activated Protein Kinase 9 (MAPK9))
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Binding Specificity
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Isoform alpha-1
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Reactivity
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Human
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Human JNK2/MAPK9 isoform alpha-1 ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificity
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This ELISA antibody pair recognizes Human JNK2.
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Storage
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4 °C
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Expiry Date
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6 months
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Target
See all JNK2 (MAPK9) ELISA Kits
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JNK2 (MAPK9)
(Mitogen-Activated Protein Kinase 9 (MAPK9))
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Alternative Name
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JNK2 (MAPK9 (MAPK9 Products)
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Synonyms
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MAPK9 ELISA Kit, JNK-55 ELISA Kit, JNK2 ELISA Kit, JNK2A ELISA Kit, JNK2ALPHA ELISA Kit, JNK2B ELISA Kit, JNK2BETA ELISA Kit, PRKM9 ELISA Kit, SAPK ELISA Kit, SAPK1a ELISA Kit, p54a ELISA Kit, p54aSAPK ELISA Kit, AI851083 ELISA Kit, Prkm9 ELISA Kit, JNK2-ALPHA1 ELISA Kit, MAP kinase 9 ELISA Kit, mitogen-activated protein kinase 9 ELISA Kit, MAP kinase 9 ELISA Kit, MAPK9 ELISA Kit, Mapk9 ELISA Kit, MPK9 ELISA Kit
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Pathways
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MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins
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