GMFB ELISA Kit

Catalog No. ABIN5690883

Product Details GMFB ELISA Kit

Target: GMFB (Glia Maturation Factor, beta (GMFB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human GMF-beta ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human GMF-beta.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for GMFB

Target: GMFB (Glia Maturation Factor, beta (GMFB))

Alternative Name: GMF-beta (GMFB Products)

Synonyms: fc25b01 ELISA Kit, zgc:56451 ELISA Kit, zgc:86831 ELISA Kit, wu:fa97c03 ELISA Kit, wu:fc25b01 ELISA Kit, GMFB ELISA Kit, DKFZp459J101 ELISA Kit, gmfb ELISA Kit, 3110001H22Rik ELISA Kit, 3110001O16Rik ELISA Kit, AI851627 ELISA Kit, C79176 ELISA Kit, D14Ertd630e ELISA Kit, GMF ELISA Kit, gmf-beta ELISA Kit, glia maturation factor, beta ELISA Kit, glia maturation factor beta ELISA Kit, Glia maturation factor beta ELISA Kit, glia maturation factor, beta L homeolog ELISA Kit, gmfb ELISA Kit, GMFB ELISA Kit, Gmfb ELISA Kit, EDI_093190 ELISA Kit, Tsp_04922 ELISA Kit, LOC100101176 ELISA Kit, gmfb.L ELISA Kit

Gene ID: 2764

UniProt: P60983