GLB1 ELISA Kit

Catalog No. ABIN5690881

Product Details GLB1 ELISA Kit

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human GLB1/Beta-galactosidase ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human GLB1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: GLB1 (Beta-galactosidase (GLB1 Products)

Synonyms: EBP ELISA Kit, ELNR1 ELISA Kit, MPS4B ELISA Kit, DER ELISA Kit, XR ELISA Kit, 0610038K04Rik ELISA Kit, 1810027P18Rik ELISA Kit, glb ELISA Kit, CG9092 ELISA Kit, Dmel\\CG9092 ELISA Kit, beta-GAL ELISA Kit, beta-Gal-1 ELISA Kit, beta-gal ELISA Kit, gal ELISA Kit, lacZ-1 ELISA Kit, AW125515 ELISA Kit, Bge ELISA Kit, Bgl ELISA Kit, Bgl-e ELISA Kit, Bgl-s ELISA Kit, Bgl-t ELISA Kit, Bgs ELISA Kit, Bgt ELISA Kit, C130097A14Rik ELISA Kit, BGAL ELISA Kit, zgc:110823 ELISA Kit, ECK0341 ELISA Kit, JW0335 ELISA Kit, GLB ELISA Kit, galactosidase beta 1 ELISA Kit, dicarbonyl and L-xylulose reductase ELISA Kit, dicarbonyl L-xylulose reductase ELISA Kit, beta-D-galactosidase ELISA Kit, beta-galactosidase ELISA Kit, beta galactosidase ELISA Kit, galactosidase, beta 1 ELISA Kit, beta-galactosidase-like ELISA Kit, GLB1 ELISA Kit, DCXR ELISA Kit, Dcxr ELISA Kit, lacZ ELISA Kit, SSP_RS00525 ELISA Kit, lacH ELISA Kit, ECL_03691 ELISA Kit, Gal ELISA Kit, Glb1 ELISA Kit, glb1 ELISA Kit, VV2_1327 ELISA Kit, LOC100056371 ELISA Kit

Gene ID: 2720

UniProt: P16278

Pathways: Glycosaminoglycan Metabolic Process