FOXO3 ELISA Kit

Catalog No. ABIN5690877

Product Details FOXO3 ELISA Kit

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human FoxO3/FKHRL1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human FoxO3.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FoxO3 (FKHRL1 (FOXO3 Products)

Synonyms: AF6q21 ELISA Kit, FKHRL1 ELISA Kit, FKHRL1P2 ELISA Kit, FOXO2 ELISA Kit, FOXO3A ELISA Kit, zgc:92176 ELISA Kit, wu:fb50g02 ELISA Kit, wu:fi33f07 ELISA Kit, Fkhrl1 ELISA Kit, Foxo3a ELISA Kit, FoxO3A ELISA Kit, 1110048B16Rik ELISA Kit, 2010203A17Rik ELISA Kit, C76856 ELISA Kit, Fkhr2 ELISA Kit, FOXO-3 ELISA Kit, FOXO3 ELISA Kit, foxo3a ELISA Kit, xfoxo3 ELISA Kit, foxO3 ELISA Kit, foxO ELISA Kit, fkhr ELISA Kit, foxo5 ELISA Kit, wu:fj65a12 ELISA Kit, zFkhr ELISA Kit, forkhead box O3 ELISA Kit, forkhead box O3A ELISA Kit, forkhead box O3 L homeolog ELISA Kit, forkhead box O3b ELISA Kit, FOXO3 ELISA Kit, foxo3a ELISA Kit, Foxo3 ELISA Kit, foxo3.L ELISA Kit, foxo3 ELISA Kit, foxO3 ELISA Kit, foxo3b ELISA Kit

Gene ID: 2309

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis