DIDO1 ELISA Kit

Catalog No. ABIN5690865

Product Details DIDO1 ELISA Kit

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human DIDO1/DIO-1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human DIDO1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for DIDO1

Target: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Alternative Name: DIDO1 (DIO-1 (DIDO1 Products)

Synonyms: 5DI ELISA Kit, TXDI1 ELISA Kit, BYE1 ELISA Kit, C20orf158 ELISA Kit, DATF-1 ELISA Kit, DATF1 ELISA Kit, DIDO2 ELISA Kit, DIDO3 ELISA Kit, DIO-1 ELISA Kit, DIO1 ELISA Kit, dJ885L7.8 ELISA Kit, 6720461J16Rik ELISA Kit, C530043I07 ELISA Kit, D130048F08Rik ELISA Kit, Datf1 ELISA Kit, Dido2 ELISA Kit, Dido3 ELISA Kit, mKIAA0333 ELISA Kit, DIDO1 ELISA Kit, datf1l ELISA Kit, fi35b04 ELISA Kit, wu:fi35b04 ELISA Kit, zgc:158157 ELISA Kit, DKFZp469J1523 ELISA Kit, bye1 ELISA Kit, dio1 ELISA Kit, datf1 ELISA Kit, dido2 ELISA Kit, dido3 ELISA Kit, dio-1 ELISA Kit, iodothyronine deiodinase 1 ELISA Kit, death inducer-obliterator 1 ELISA Kit, death inducer-obliterator 1 L homeolog ELISA Kit, DIO1 ELISA Kit, DIDO1 ELISA Kit, Dido1 ELISA Kit, dido1.L ELISA Kit, dido1 ELISA Kit

Gene ID: 11083

UniProt: Q9BTC0

Pathways: Hormone Activity