Dihydrofolate Reductase ELISA Kit

Catalog No. ABIN5690864

Product Details Dihydrofolate Reductase ELISA Kit

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human DHFR ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human DHFR.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR (DHFR Products)

Synonyms: DHFR ELISA Kit, F10_orf160 ELISA Kit, ECK0049 ELISA Kit, JW0047 ELISA Kit, tmrA ELISA Kit, cb595 ELISA Kit, zgc:86637 ELISA Kit, DDBDRAFT_0187132 ELISA Kit, DDBDRAFT_0230113 ELISA Kit, DDB_0187132 ELISA Kit, DDB_0230113 ELISA Kit, CG14887 ELISA Kit, Dmel\\CG14887 ELISA Kit, dhfr ELISA Kit, DHFRP1 ELISA Kit, DYR ELISA Kit, 8430436I03Rik ELISA Kit, AA607882 ELISA Kit, AI662710 ELISA Kit, AW555094 ELISA Kit, Dhfr1 ELISA Kit, confers resistance to sulfonamides ELISA Kit, dihydrofolate reductase ELISA Kit, dihydrofolate reductase DhfR ELISA Kit, Dihydrofolate reductase ELISA Kit, dhfr ELISA Kit, DHFR ELISA Kit, dhfR ELISA Kit, folA ELISA Kit, dfrA ELISA Kit, LOC707382 ELISA Kit, CNJ01940 ELISA Kit, DDB_G0286755 ELISA Kit, Dhfr ELISA Kit, Mesil_0096 ELISA Kit, Arch_0408 ELISA Kit, Fbal_3367 ELISA Kit, Ilyop_0482 ELISA Kit, Lbys_2007 ELISA Kit, Riean_0733 ELISA Kit, Mrub_1855 ELISA Kit, Poras_0766 ELISA Kit, Halhy_3552 ELISA Kit, Mesop_3614 ELISA Kit, LOC609048 ELISA Kit

Gene ID: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases