Cyclin D1 ELISA Kit

Catalog No. ABIN5690860

Product Details Cyclin D1 ELISA Kit

Target: Cyclin D1 (CCND1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Cyclin D1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Cyclin D1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1 (CCND1 Products)

Synonyms: BCL1 ELISA Kit, D11S287E ELISA Kit, PRAD1 ELISA Kit, U21B31 ELISA Kit, AI327039 ELISA Kit, CycD1 ELISA Kit, Cyl-1 ELISA Kit, bcl-1 ELISA Kit, cD1 ELISA Kit, cb161 ELISA Kit, cycd1 ELISA Kit, etID37810.7 ELISA Kit, fb52e01 ELISA Kit, fc45c08 ELISA Kit, fc83a12 ELISA Kit, id:ibd1198 ELISA Kit, wu:fb52e01 ELISA Kit, wu:fc45c08 ELISA Kit, wu:fc83a12 ELISA Kit, ccnd1b ELISA Kit, prad1 ELISA Kit, ccnd1 ELISA Kit, ccnd1a ELISA Kit, bcl1 ELISA Kit, d11s287e ELISA Kit, u21b31 ELISA Kit, CCND1 ELISA Kit, cyclin D1 ELISA Kit, cyclin D1 S homeolog ELISA Kit, cyclin D1 L homeolog ELISA Kit, CCND1 ELISA Kit, Ccnd1 ELISA Kit, ccnd1 ELISA Kit, ccnd1.S ELISA Kit, ccnd1.L ELISA Kit

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling