Crk ELISA Kit

Catalog No. ABIN5690859

Product Details Crk ELISA Kit

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Crk ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Crk.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for Crk

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Alternative Name: Crk (CRK Products)

Synonyms: CG1587 ELISA Kit, CRK ELISA Kit, D-CRK ELISA Kit, D-Crk ELISA Kit, DCrk ELISA Kit, Dcrk ELISA Kit, Dmel\\CG1587 ELISA Kit, crk ELISA Kit, dCRK ELISA Kit, dCrk ELISA Kit, fb34h10 ELISA Kit, fc05a01 ELISA Kit, fi19g05 ELISA Kit, wu:fb34h10 ELISA Kit, wu:fc05a01 ELISA Kit, wu:fc54a04 ELISA Kit, wu:fi19g05 ELISA Kit, zgc:100936 ELISA Kit, CRKII ELISA Kit, p38 ELISA Kit, Crko ELISA Kit, crk2 ELISA Kit, crkII ELISA Kit, v-crk ELISA Kit, P38C-CRK ELISA Kit, Crk-I ELISA Kit, Crk-II ELISA Kit, Crk-III ELISA Kit, Crk3 ELISA Kit, CrkIII ELISA Kit, c-Crk ELISA Kit, Crk oncogene ELISA Kit, v-crk avian sarcoma virus CT10 oncogene homolog ELISA Kit, CRK proto-oncogene, adaptor protein ELISA Kit, v-crk avian sarcoma virus CT10 oncogene homolog L homeolog ELISA Kit, v-crk sarcoma virus CT10 oncogene homolog ELISA Kit, Crk ELISA Kit, crk ELISA Kit, CRK ELISA Kit, crk.L ELISA Kit

Gene ID: 1398

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor