CDX4 ELISA Kit
CDX4
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Target
See all CDX4 products
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CDX4
(Caudal Type Homeobox 4 (CDX4))
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Reactivity
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Human
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Detection Method
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Colorimetric
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Method Type
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Sandwich ELISA
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Application
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ELISA
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Purpose
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Human CDX4 ELISA Kit.
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Sample Type
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificity
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This ELISA antibody pair recognizes Human CDX4.
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Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plate
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Pre-coated
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Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Storage
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4 °C
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Expiry Date
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6 months
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Target
See all CDX4 products
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CDX4
(Caudal Type Homeobox 4 (CDX4))
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Alternative Name
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CDX4 (CDX4 Products)
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Synonyms
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Xcad3 ELISA Kit, Xcad-3 ELISA Kit, Xtcad3 ELISA Kit, MGC68909 ELISA Kit, MGC130769 ELISA Kit, CDXB ELISA Kit, CDX4 ELISA Kit, CHUNP6923 ELISA Kit, cad1 ELISA Kit, cb546 ELISA Kit, cdx1 ELISA Kit, id:ibd1215 ELISA Kit, id:ibd2812 ELISA Kit, id:ibd5107 ELISA Kit, kgg ELISA Kit, kugelig ELISA Kit, Cdx-3 ELISA Kit, Cdx-4 ELISA Kit, Cdx3 ELISA Kit, RGD1561529 ELISA Kit, cad3 ELISA Kit, cdx4 ELISA Kit, caudal type homeobox 4 ELISA Kit, caudal type homeo box 4 ELISA Kit, caudal type homeobox 4 L homeolog ELISA Kit, CDX4 ELISA Kit, cdx4 ELISA Kit, Cdx4 ELISA Kit, cdx4.L ELISA Kit
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Gene ID
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1046
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UniProt
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O14627
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