CDK5R1 ELISA Kit

Catalog No. ABIN5690848

Product Details CDK5R1 ELISA Kit

Target: CDK5R1 (Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human CDK5 Activator 1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human CDK5 Activator 1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for CDK5R1

Target: CDK5R1 (Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (p35) (CDK5R1))

Alternative Name: CDK5 Activator 1 (CDK5R1 Products)

Synonyms: CDK5P35 ELISA Kit, CDK5R ELISA Kit, NCK5A ELISA Kit, p23 ELISA Kit, p25 ELISA Kit, p35 ELISA Kit, p35nck5a ELISA Kit, cdk5r1 ELISA Kit, zgc:92814 ELISA Kit, CDK5R1 ELISA Kit, Cdk5r ELISA Kit, P25/P35 ELISA Kit, D11Bwg0379e ELISA Kit, Xp35.1 ELISA Kit, cyclin dependent kinase 5 regulatory subunit 1 ELISA Kit, cyclin-dependent kinase 5, regulatory subunit 1b (p35) ELISA Kit, cyclin-dependent kinase 5 regulatory subunit 1 ELISA Kit, S100 calcium binding protein A9 ELISA Kit, cyclin-dependent kinase 5, regulatory subunit 1 (p35) ELISA Kit, cyclin-dependent kinase 5, regulatory subunit 1 (p35) L homeolog ELISA Kit, CDK5R1 ELISA Kit, cdk5r1b ELISA Kit, Cdk5r1 ELISA Kit, S100A9 ELISA Kit, cdk5r1.L ELISA Kit

Target Type: Viral Protein

Gene ID: 8851

UniProt: Q15078

Pathways: Stem Cell Maintenance, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity