beta Arrestin 1 ELISA Kit

Catalog No. ABIN5690830

Product Details beta Arrestin 1 ELISA Kit

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human beta-Arrestin-1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human beta-Arrestin-1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for beta Arrestin 1

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Alternative Name: beta-Arrestin-1 (ARRB1 Products)

Synonyms: arrb1 ELISA Kit, MGC83787 ELISA Kit, arb1 ELISA Kit, arr1 ELISA Kit, barr1 ELISA Kit, betaarr1 ELISA Kit, 1200006I17Rik ELISA Kit, AW208571 ELISA Kit, G430100A01Rik ELISA Kit, ARB1 ELISA Kit, ARR1 ELISA Kit, BARRES ELISA Kit, arrestin beta 1 ELISA Kit, arrestin, beta 1 ELISA Kit, arrestin beta 1 L homeolog ELISA Kit, beta-arrestin 1 ELISA Kit, putative beta-arrestin 1 ELISA Kit, ARRB1 ELISA Kit, arrb1 ELISA Kit, arrb1.L ELISA Kit, CpipJ_CPIJ016988 ELISA Kit, CpipJ_CPIJ017114 ELISA Kit, Smp_122900 ELISA Kit, Smp_126080 ELISA Kit, Smp_176980 ELISA Kit, Smp_152290 ELISA Kit, Arrb1 ELISA Kit

Gene ID: 408

UniProt: P49407

Pathways: Positive Regulation of Peptide Hormone Secretion, Nuclear Hormone Receptor Binding, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction