CLMP ELISA Kit

Catalog No. ABIN5690824

Product Details CLMP ELISA Kit

Target: CLMP (CXADR-Like Membrane Protein (CLMP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ASAM/ACAM ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human ASAM.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for CLMP

Target: CLMP (CXADR-Like Membrane Protein (CLMP))

Alternative Name: ASAM (ACAM (CLMP Products)

Synonyms: ACAM ELISA Kit, ASAM ELISA Kit, CSBM ELISA Kit, CSBS ELISA Kit, Asam ELISA Kit, Ol16 ELISA Kit, 9030425E11Rik ELISA Kit, ASP5 ELISA Kit, AW557819 ELISA Kit, CXADR like membrane protein ELISA Kit, CXADR-like membrane protein ELISA Kit, CLMP ELISA Kit, Clmp ELISA Kit

Gene ID: 71566

UniProt: Q8R373