ACPP ELISA Kit

Catalog No. ABIN5690815

Product Details ACPP ELISA Kit

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ACPP ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human ACPP.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACPP (ACPP Products)

Synonyms: 5'-NT ELISA Kit, ACP-3 ELISA Kit, ACP3 ELISA Kit, PAP ELISA Kit, AMAP2 ELISA Kit, CENTB3 ELISA Kit, DDEF2 ELISA Kit, PAG3 ELISA Kit, Pap-alpha ELISA Kit, SHAG1 ELISA Kit, A030005E02Rik ELISA Kit, FRAP ELISA Kit, Lap ELISA Kit, Ppal ELISA Kit, Acpp11 ELISA Kit, RNACPP11 ELISA Kit, pap ELISA Kit, ACPP ELISA Kit, acp-3 ELISA Kit, acp3 ELISA Kit, acpt ELISA Kit, acid phosphatase, prostate ELISA Kit, ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 ELISA Kit, prostatic acid phosphatase ELISA Kit, prostatic acid phosphatase, putative ELISA Kit, acid phosphatase, prostate S homeolog ELISA Kit, ACPP ELISA Kit, ASAP2 ELISA Kit, Acpp ELISA Kit, CpipJ_CPIJ004002 ELISA Kit, Smp_016640 ELISA Kit, acpp.S ELISA Kit

Gene ID: 55

UniProt: P15309

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process