CER1 ELISA Kit

Catalog No. ABIN5690782

Product Details CER1 ELISA Kit

Target: CER1 (Cerberus 1 Homolog (Xenopus Laevis) (CER1))

Reactivity: Chicken

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Chicken Caronte ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Chicken Caronte.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for CER1

Target: CER1 (Cerberus 1 Homolog (Xenopus Laevis) (CER1))

Alternative Name: Caronte (CER1 Products)

Synonyms: DAND4 ELISA Kit, CAR ELISA Kit, cer ELISA Kit, cer-1 ELISA Kit, dand4 ELISA Kit, tCerberus ELISA Kit, xcer-1 ELISA Kit, xcer1 ELISA Kit, Cerl ELISA Kit, Cerl1 ELISA Kit, Cerr1 ELISA Kit, RGD1563046 ELISA Kit, cerberus 1, DAN family BMP antagonist ELISA Kit, cerberus 1, DAN family BMP antagonist S homeolog ELISA Kit, CER1 ELISA Kit, cer1.S ELISA Kit, Cer1 ELISA Kit

Gene ID: 395623

UniProt: Q9PUK2

Pathways: Maintenance of Protein Location